Fig. 2. Markers of proliferation and apoptosis. (A) Proliferating cell nuclear antigen (PCNA) antibody staining: marker of cell regeneration. Arrows show positive cells. All images were taken at a magnification of 20x. Scale bar: 100 μm. (B) Quantification of renal cells positively stained for PCNA. (C) Cleaved-caspase 3 (cCasp3) antibody staining: marker of apoptosis. Arrows show positive cells. All images were taken at a magnification of 20x. Scale bar: 100 μm. (D) Quantification of renal cells positively stained for cCasp3. All values are means ± SD. ns p>0.05, ***p<0.001 and ****p<0.0001. Wild type (WT) and Trpc6^-/- sham groups included n=5 kidney samples each. WT and Trpc6^-/- UUO-treated groups encompassed n=12 (WT) and n=11 (Trpc6^-/-) kidney samples. ns, not significant.